Glen or Glenda

Director

Scientist

Glen / Glenda (as Daniel Davis)

Inspector Warren

Dr. Alton / Narrator

Barbara

Alan / Anne

Johnny (as Charles Crafts)

Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)

Man with Hat and Receding Hairline (uncredited)

Woman in Nightmare (uncredited)

The Devil / Glen's Father (uncredited)